| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium tertium | GGAAGCTCTGACACAACAGC | GCCAAGTGCTAGTACCCCTG | 59.13 | 60.11 | 237 | 176 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium tertium | GGAAGCTCTGACACAACAGC | GCTAGTACCCCTGCAGAAGC | 59.13 | 60.18 | 230 | 176 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium tertium | GGAAGCTCTGACACAACAGC | AGTGCTAGTACCCCTGCAG | 59.13 | 58.40 | 233 | 176 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium tertium | GGAAGCTCTGACACAACAGC | CCAAGTGCTAGTACCCCTGC | 59.13 | 60.11 | 236 | 168 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium tertium | GGGTGAGGAGTTAACAGGAGG | ACTTCCTTCTGTGCGGAGG | 59.44 | 59.33 | 269 | 167 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium tertium | CAGGGCTAAAGGCAACAGC | TCCAATGATCCTGGAGCTGG | 59.11 | 59.16 | 190 | 165 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium tertium | AGGGGTGAGGAGTTAACAGG | ACTTCCTTCTGTGCGGAGG | 58.34 | 59.33 | 271 | 156 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium tertium | CAGGGCTAAAGGCAACAGC | TTCCAATGATCCTGGAGCTGG | 59.11 | 59.79 | 191 | 154 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium tertium | GGGGAATGCGAAATTGGTGG | TGCATTCTCGCTCCTCCAG | 59.82 | 59.48 | 173 | 148 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium tertium | AGCAGGATGTACAACGGGTG | CGTCATTTGCTCTCGCTGC | 60.04 | 59.94 | 296 | 146 |
100.00%
|
100.00%
|